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Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
 

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples

Article DOI: 10.3791/51116-v • 14:39 min • April 21st, 2014
April 21st, 2014 •
1, 2, 2, 1, 1, 1, 2, 1
1Department NeuroFarBa, Division of Pharmacology, University of Florence, 2Department of Clinical and Experimental Medicine, Division of Physiology, University of Florence

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Current knowledge on the cellular basis of cardiac diseases mostly relies on studies on animal models. Here we describe and validate a novel method to obtain single viable cardiomyocytes from small surgical samples of human ventricular myocardium. Human ventricular myocytes can be used for electrophysiological studies and drug testing.

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Isolation Functional Characterization Human Ventricular Cardiomyocytes Surgical Samples Remodeling Processes Cell Structure Excitation Contraction Coupling Membrane Ion Currents Arrhythmogenic Risk Contractile Alterations Systolic Dysfunction Diastolic Dysfunction Cardiac Patients Myocyte Function Cardiac Diseases Animal Models Protocol Viable Myocytes Ventricular Myocardium Cardiac Surgery Operations Electrophysiological Measurements Intracellular Calcium Measurements Single Cell Measurements Cellular And Molecular Basis Therapeutic Targets Translational Value
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