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MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
 

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Article DOI: 10.3791/51434-v • 09:59 min • March 5th, 2014
March 5th, 2014 •
1, 1,2
1Department of Chemistry, University of Wisconsin- Madison, 2School of Pharmacy, University of Wisconsin- Madison

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Mass spectrometric imaging (MSI) is a powerful tool that can be used to discover and identify various chemical species in intact tissues, preserving the compounds in their native environments, which can provide new insights into biological processes. Herein a MSI method developed for the analysis of small molecules is described. 

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MALDI-Mass Spectrometric Imaging Metabolites Medicago Truncatula Root Nodules Small Molecules Tissue Extracts Homogenization Metabolic Pathways Mass Spectrometry Analytical Tool Spatial Information Intact Slices Biological Tissue Samples Proteins Peptides Lipids Endogenous Metabolites Matrix-assisted Laser Desorption/ionization (MALDI) Untargeted Metabolomics MSI Experiments Legume Roots Image Acquisition Matrix Application Techniques MS Images Analysis Identification
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