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Immunology and Infection

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牛痘病毒记者量化病毒功能在基因表达的各个阶段
 
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牛痘病毒记者量化病毒功能在基因表达的各个阶段

Article DOI: 10.3791/51522-v 10:48 min May 15th, 2014
May 15th, 2014

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我们描述了一种荧光报告牛痘病毒,使病毒的感染性和基因表达的实时测量通过的光谱不同的荧光报告基团的阶段特异性表达的用法。我们详细的板基础的方法准确地确定在哪个病毒复制受到影响响应小分子抑制阶段。

Tags

免疫学,第87,牛痘;痘病毒;感染;病毒与宿主的相互作用;屏幕;抑制剂;基因表达;细胞生物学;荧光;抗病毒药物;记者的mCherry,金星,TagBFP
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