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An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
 

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

Article DOI: 10.3791/51530-v • 09:33 min • June 19th, 2014
June 19th, 2014 •
1, 2, 1
1Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich, 2Institut de Recherche pour le Développement (UMR 232), Centre National de la Recherche Scientifique (ERL 5300), Université de Montpellier II

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We provide here an efficient and reliable protocol for immunostaining, Fluorescence in situ Hybridization, DNA staining followed by quantitative, high-resolution imaging in whole-mount Arabidopsis thaliana ovules. This method was successfully used to analyze chromatin modifications and nuclear architecture.

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Keywords: Arabidopsis Ovule Single-cell Analysis Chromatin Modification Nuclear Architecture Immunodetection Fluorescence In Situ Hybridization (FISH) Confocal Laser Scanning Microscopy (CLSM)
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