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Bioengineering

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JoVE Journal Bioengineering
Construction and Characterization of a Novel Vocal Fold Bioreactor
 

Construction and Characterization of a Novel Vocal Fold Bioreactor

Article DOI: 10.3791/51594-v • 11:11 min • August 1st, 2014
August 1st, 2014 •
1, 2, 2, 1,2
1Biomedical Engineering Program, University of Delaware, 2Department of Materials Science and Engineering, Delaware Biotechnology Institute, University of Delaware

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A novel vocal fold bioreactor capable of delivering physiologically relevant, vibratory stimulation to cultured cells is constructed and characterized. This dynamic culture device, when combined with a fibrous poly(ε-caprolactone) scaffold, creates a vocal fold-mimetic environment that modulates the behaviors of mesenchymal stem cells.

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Vocal Fold Bioreactor In Vitro Engineering Mechanical Conditions Dynamic Environment Vibratory Stimulations Fundamental Phonation Frequencies Function Generator Power Amplifier Speaker Selector Vibration Chambers Silicone Membrane Acrylic Blocks Cell-laden Scaffold Oscillating Air Vibration Modules Anti-humidity Chamber Long-term Operation Cell Culture Incubator Laser Doppler Vibrometer (LDV) Dynamic Culture Device Cellular Constructs Sinusoidal Vibrations Mid-membrane Displacement Mesenchymal Stem Cells
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