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EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
 

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Article DOI: 10.3791/51611-v • 06:01 min • November 26th, 2014
November 26th, 2014 •
1, 1, 1
1Department of Biotechnology, Delft University of Technology

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The goal of this protocol is to use electron paramagnetic resonance (EPR) monitored redox titrations to identify different cofactors of Saccharomyces cerevisiae Nar1. Redox titrations offer a very robust way to obtain midpoint potentials of different redox active cofactors in enzymes and proteins.

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Keywords: EPR Redox Titration Saccharomyces Cerevisiae Nar1 Cofactors Electron Paramagnetic Resonance Midpoint Potential Voltammetry Redox Mediators Nernst Equation
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