Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Generation of Genomic Deletions in Mammalian Cell …
JoVE Journal
Biology

Questo Contenuto è ad Accesso gratuito.

JoVE Journal Biology
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
 

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Article DOI: 10.3791/52118-v • 09:40 min • January 3rd, 2015
January 3rd, 2015 •
*1,2,3, *1, 1,2,3,4
1Harvard Medical School, 2Division of Hematology/Oncology, Boston Children's Hospital, 3Department of Pediatric Oncology, Dana-Farber Cancer Institute, 4Howard Hughes Medical Institute
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

CRISPR/Cas9 is a robust system to produce disruption of genes and genetic elements. Here we describe a protocol for the efficient creation of genomic deletions in mammalian cell lines using CRISPR/Cas9.

Tags

Genomic Deletions CRISPR/Cas9 Genome Engineering Single Guide RNA (sgRNA) Double-strand Breaks (DSBs) Non-homologous End Joining (NHEJ) Loss-of-function Studies Mammalian Cell Lines
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter