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Anaerobic Growth and Maintenance of Mammalian Cell Lines
 

Anaerobic Growth and Maintenance of Mammalian Cell Lines

Article DOI: 10.3791/58049-v • 07:15 min • July 21st, 2018
July 21st, 2018 •
1, 1, 1, 1
1Department of Microbiology and Immunology, Midwestern University

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Here, we describe a new method that enables the anaerobic long-term cultivation of established cell lines. The maximum survival time that was tested is 17 days. This method is suitable for the testing of cytotoxic agents and exploring the physiology of anoxically replicating cells.

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Keywords: Anaerobic Growth Mammalian Cell Lines Anoxic Cultivation Oxygen Toxicity Anaerobic Respiration Metabolism Stem Cell Cultivation Oxygen Contamination Nitrite DMEM Vacuum Anaerobic Chamber Oxygen Probe Trypsin Cell Counting Cell Viability
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