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A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity
 

A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl

Article DOI: 10.3791/52419-v • 05:20 min • November 28th, 2014
November 28th, 2014 •
1, 1, 1
1Cancer Immunology Program, Peter MacCallum Cancer Centre

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We describe a simple, quantitative colorimetric assay that specifically measures the proteolytic activity of human, mouse or rat Granzyme B (GzmB). This protocol can be easily adapted for determining protease activity of other granule serine proteases by the hydrolysis of other synthetic peptide substrates with an appropriate recognition sequence.

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Colorimetric Assay Granzyme B Proteolytic Activity Boc-Ala-Ala-Asp-S-Bzl Serine Protease Target Cell Apoptosis Cytotoxic T Lymphocytes Natural Killer Cells Pathophysiology Caspases Peptide Thioester Mammalian Serine Protease Lysates Activated NK Cells CTL Recombinant Proteases Pro-apoptotic Activity Mammalian Lymphocytes
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