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Bioengineering

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JoVE Journal Bioengineering
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement (EFIRM)
 

Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement (EFIRM)

Article DOI: 10.3791/52439-v • 11:02 min • January 23rd, 2015
January 23rd, 2015 •
1, 1, 2, 1
1School of Dentistry, University of California, Los Angeles, 2School of Medicine, Clinical Nutrition, University of California, Los Angeles

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Exosomes are microvesicular structures found within biofluids that potentially carry important disease discriminatory biomarkers. Here, a novel method is used to specifically extract exosomes and rapidly test the exosomal cargo for both RNA/protein targets following the disruption of exosomes using non-uniform electric cyclic square waves.

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Exosomal Biomarker Detection Electric Field-induced Release And Measurement (EFIRM) Intercellular Communication Exosomal Analysis Cargo Extraction RNA/protein Content Anti-human CD63 Specific Antibody Magnetic Microparticle Low-voltage Electric Cyclic Square Waves (CSW) Vesicular Membrane Disruption Cargo Unloading Molecular Content Quantification Lysis Buffer-free Extraction RNA And Protein Biomarker Targets Surface Marker-based Extraction Transmission Electron Microscopy (TEM)
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