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Three-dimensional Co-culture Model for Tumor-stromal Interaction
 

Three-dimensional Co-culture Model for Tumor-stromal Interaction

Article DOI: 10.3791/52469-v • 08:39 min • February 2nd, 2015
February 2nd, 2015 •
1,2, 1,3, 4, 5, 1
1Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, 2Department of Clinical Laboratory, Graduate School of Medicine, The University of Tokyo, 3Division for Health Service Promotion, The University of Tokyo, 4Department of Biochemistry, Nihon University School of Dentistry, 5Department of Biochemistry, Ohu University School of Pharmaceutical Sciences

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Here we present a protocol to co-culture in three-dimensions, which is useful for investigating multicellular interactions and extracellular matrix-dependent modulation of cancer cell behavior. In this experimental model, cancer cells are cultured on collagen gels embedded with human cancer-associated fibroblasts.

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Three-dimensional Co-culture Model Tumor-stromal Interaction Cancer Progression Malignant Cells Tumor Microenvironment Fibroblasts Immune Cells Vascular Endothelial Cells Pericytes Bone-marrow-derived Cells Extracellular Matrix (ECM) Cancer-associated Fibroblasts (CAFs) Pro-tumorigenic Role Soluble Factors Angiogenesis ECM Remodeling Experimental Models Cancer Cell Survival Proliferation Migration Invasion Two-dimensional Monocellular Cultures Monolayer Tissue Cultures Boyden Chamber Assays Physiological Conditions Pathological Conditions Diseased Organ Multicellular Cultures Intercellular Communication ECM-dependent Modulation
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