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Developmental Biology

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JoVE Journal Developmental Biology
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
 

Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing

Article DOI: 10.3791/52489-v • 09:36 min • February 25th, 2015
February 25th, 2015 •
1, 1, 2, 1, 2, 1
1Biomedical Sciences Program, Midwestern University, 2Department of Microbiology & Immunology, Midwestern University

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Zebrafish keratocytes migrate in cell sheets from explants and provide an in vitro model for the study of the mechanisms of collective cell migration in the context of epithelial wound healing. These protocols detail an effective way to establish primary explant cultures for use in collective cell migration assays.

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Keywords: Zebrafish Keratocyte Explant Collective Cell Migration Reepithelialization Cutaneous Wound Healing Single Cell Migration Actin-rich Lamellae Actin Cable Epithelial To Mesenchymal Transition Gene Expression Mutant Transgenic In Vitro Model
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