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Immunology and Infection

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JoVE Journal Immunology and Infection
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
 

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Article DOI: 10.3791/52739-v • 07:12 min • April 16th, 2015
April 16th, 2015 •
1, 1
1Department of Microbiology and Immunology, The Chicago Medical School, Rosalind Franklin University of Medicine and Science

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Naïve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of naïve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals.

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Mouse Na& 239;ve CD4+ T Cell Isolation In Vitro Differentiation T Cell Subsets Antigen Inexperienced Expansion Effector Subsets T Cell Receptor (TCR) Cognate Antigen MHC Class II Cytokine Signals Environment TCR Activation Effector Fate Cytokine Combinations Hallmark Cytokine Transcription Factor Expression Differentiation Experiments Evaluation Of Targets CD4+ T Helper Subsets Mediators Signaling Agonists Antagonists Cytokines Isolation Protocol Polarizing Na& 239;ve Cells
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