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JoVE Journal Neuroscience
Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes
 

Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes

Article DOI: 10.3791/53113-v • 09:13 min • December 11th, 2015
December 11th, 2015 •
1, 1, 2, 3,4, 1
1Department of Clinical and Experimental Medicine, Linköping University, 2Neurophysiology Research Center, Shahed University, 3Cellular and Molecular Research Center, Iran University of Medical Sciences, 4School of Advanced Technologies in Medicine, Iran University of Medical Sciences

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Astrocytes in the CNS change their functional and structural properties in response to harmful stimuli. This report presents a protocol for assessment of three-dimensional astrocyte morphology in diseased conditions or after therapeutic interventions.

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Three-dimensional Confocal Morphometric Analysis Astrocytes Glial Cells Central Nervous System Reactive Astrogliosis Assessment Morphological Properties Quantification Surface Area Volume Astrocyte Territory Branches Cell Body Nucleus Total Length Number Of Branches Fluorescence Immunoreactivity Antibodies Astrocyte Detection Astrocyte Density Confocal Microscopic Images Image Analysis Software Volocity 6.3 Measurements Rat Brain Tissue Amyloid Beta1-40 (Aβ1-40) Therapeutic Intervention In Vivo Conditions
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