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A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis
 

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

Article DOI: 10.3791/53407-v • 11:04 min • December 19th, 2015
December 19th, 2015 •
*1, *2, 1, 1
1Friedrich Miescher Laboratory, Max Planck Society, 2Nencki Institute of Experimental Biology, Polish Academy of Sciences
* These authors contributed equally

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The molecular mechanisms of the decondensation of highly compacted mitotic chromatin are ill-defined. We present a cell-free assay based on mitotic chromatin clusters isolated from HeLa cells and Xenopus laevis egg extract that faithfully reconstitutes the decondensation process in vitro.

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Keywords: Cell-free Assay Chromatin Decondensation Mitosis Mitotic Exit Nuclear Envelope X. Laevis Egg Extract HeLa Cells Molecular Events Chromatin Condensation Chromosome Structure
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