Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Purification and Refolding to Amyloid Fibrils of…
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Biology
Purification and Refolding to Amyloid Fibrils of (His)6-tagged Recombinant Shadoo Protein Expressed as Inclusion Bodies in E. coli
 

Purification and Refolding to Amyloid Fibrils of (His)6-tagged Recombinant Shadoo Protein Expressed as Inclusion Bodies in E. coli

Article DOI: 10.3791/53432-v • 09:43 min • December 19th, 2015
December 19th, 2015 •
1, 1, 1, 1
1Virologie et Immunologie Moléculaires, INRA

Capitoli

Riepilogo
Automatic Translation

A two-step chromatographic method is described for the purification of recombinant Shadoo protein expressed as inclusion bodies in Escherichia coli, as well as a protocol to fibrillate purified Shadoo into amyloid structures.

Tags

Keywords: Recombinant Shadoo Protein Inclusion Bodies Purification Affinity Chromatography Size Exclusion Chromatography Amyloid Fibrils Prion Diseases
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter