Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / High Throughput Quantitative Expression Screening …
JoVE Journal
Biology

This Contenuto is Open Access.

JoVE Journal Biology
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
 

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Article DOI: 10.3791/51464-v • 12:16 min • July 30th, 2014
July 30th, 2014 •
1, 2, 1, 1
1Architecture et Fonction des Macromolécules Biologiques (AFMB), Aix-Marseille Université, 2iBiTec-S, Service d'Ingénierie Moléculaire des Protéines (SIMOPRO), Commissariat à l'énergie atomique et aux énergies alternatives (CEA) Saclay, France

Capitoli

Riepilogo
Automatic Translation

A protocol for the quantitative, high throughput expression screening and analytical purification of fusion proteins from small-scale Escherichia coli cultures is described and applied to the expression of disulfide-rich animal venom protein targets.

Tags

Keywords: High Throughput Quantitative Expression Screening Recombinant Disulfide-rich Venom Proteins E. Coli Structural Genomics DsbC Disulfide Bond Formation Protein Production Therapeutic Drug Leads Venom Proteins Mass Spectrometry Micro-assays
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter