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A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
 

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Article DOI: 10.3791/53513-v • 12:18 min • April 17th, 2016
April 17th, 2016 •
*1, *1, 1, 1, 2, 1
1Division of Applied Life Science (BK21 plus program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 2Biotechnology Research Institute, Chinese Academy of Agricultural Sciences
* These authors contributed equally

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This article describes the methods for screening the genes controlling plasmodesmal permeability and hence auxin gradient during tropic response. This includes the measurement of the degree of tropic response in hypocotyl of Arabidopsis thaliana and checking plasmodesmal permeability by 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) loading and finally callose level assessment.

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Callose-mediated Plasmodesmal Gating Tropic Response Genes Cell-to-cell Signaling Plant Developmental Biology Arabidopsis Etiolated Hypocotyls GSL8 Glucose Synthase Murashige And Skoog Medium Sterilization Sodium Hypochlorite Micropipette Tip Water Seed Arrangement
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