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Journal / Developmental Biology / Using Light Sheet Fluorescence Microscopy to…
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Developmental Biology

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JoVE Journal Developmental Biology
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
 

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Article DOI: 10.3791/53966-v • 13:01 min • April 10th, 2016
April 10th, 2016 •
*1, *1, 1, 1, 1,2,3, 1
1Max Planck Institute of Molecular Cell Biology and Genetics, 2HHMI Janelia Research Campus, 3Berlin Institute of Medical Systems Biology of the Max Delbrück Center
* These authors contributed equally

Capitoli

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Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

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Light Sheet Fluorescence Microscopy Zebrafish Eye Development Experimental Protocol Multiview Data Set Fiji Morphogenetic Events Animal Development Spacial And Temporal Resolution Low Phototoxicity Fast Acquisition Timing Various Angles Views Signal Degradation Axis Degradation Fluorescent Microspheres Three Dimensional Volume Reconstruction Isotropic Resolution Developmental Biology Neuroscience Model Organisms
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