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Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells
 

Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells

Article DOI: 10.3791/54353-v • 08:17 min • December 9th, 2016
December 9th, 2016 •
1,2, 2, 1,2, 2, 3, 2
1Bell Research Center for Reproductive Health and Cancer, 2Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 3Department of Cancer Biology, Nagoya University Graduate School of Medicine

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To clarify the multistep process of peritoneal dissemination of ovarian carcinoma, the current study presents a murine experimental model of original tumor development and peritoneal metastasis via orthotopic inoculation with these tumor cells.

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Murine Experimental Model Original Tumor Development Peritoneal Metastasis Orthotopic Inoculation Ovarian Carcinoma Cells Gynecologic Oncological Field Tumor Biology Peritoneal Dissemination Retroperitoneal Approach Human Epithelial Ovarian Carcinoma ES2 Cells RPMI-1640 Medium FBS PenStrep Trypsin/EDTA Logarithmic Growth Phase PBS Wash Serum-containing Growth Medium Centrifuge Epithelial Ovarian Carcinoma (EOC)
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