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Developmental Biology

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JoVE Journal Developmental Biology
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
 

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Article DOI: 10.3791/54372-v • 11:00 min • December 16th, 2016
December 16th, 2016 •
1,2,3, 4, 1,2,3, 1,3,5, 1,3
1Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 2The Graduate School of Biomedical Science, Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 4Center for Neuroscience and Cell Biology, University of Coimbra, 5Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai

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The protocol described here details the induction of a hemogenic program in mouse embryonic fibroblasts via overexpression of a minimal set of transcription factors. This technology may be translated to the human system to provide platforms for future study of hematopoiesis, hematologic disease, and hematopoietic stem cell transplant.

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Keywords: Fibroblast Reprogramming Transcription Factors Hemogenic Induction Hematopoietic Precursor Cells HSCs Multi-lineage Differentiation Gelatin Coating Virus Transduction Doxycycline Induction Hematopoietic Culture Medium Cell Dissociation Cell Counting Cell Plating
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