Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Flow-sorting and Exome Sequencing of the…
JoVE Journal
Genetics

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Genetics
Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
 

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Article DOI: 10.3791/54399-v • 08:53 min • June 10th, 2017
June 10th, 2017 •
1, 2, 3, 4, 5, 6
1Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility, Weill Cornell Medical College, 3Department of Laboratory Medicine, University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 6Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center

Capitoli

Riepilogo
Automatic Translation

Here, we describe a combined flow cytometric cell sorting and low-input, next-generation library construction protocol designed to produce high-quality, whole-exome data from the Hodgkin Reed-Sternberg (HRS) cells of classical Hodgkin lymphoma (CHL).

Tags

Keywords: Flow-sorting Exome Sequencing Reed-Sternberg Cells Classical Hodgkin Lymphoma CHL HRS Cells Next-generation Sequencing Tumor-derived DNA Diagnosis Classification Prognosis Therapy Flow Cytometry Antibody Cocktail Thawing Medium Sorting Medium
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter