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Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice
 

Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice

Article DOI: 10.3791/54555-v • 09:32 min • October 23rd, 2016
October 23rd, 2016 •
*1, *1,2, 1,3
1Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, 2Department of Bioengineering, University of Colorado Anschutz Medical Campus, 3Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus
* These authors contributed equally

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Methods are demonstrated for the isolation of sinoatrial node myocytes (SAMs) from adult mice for patch clamp electrophysiology or imaging studies. Isolated cells can be used directly or can be maintained in culture to permit expression of proteins of interest, such as genetically encoded reporters.

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Isolation Culture Functional Characterization Sinoatrial Node Myocytes Adult Mice Electrophysiological Experiments Imaging Experiments Cardiac Pacemaking Mechanisms Ion Currents Intracellular Calcium Release Written Methods Solutions Fur Removal Ribcage Transection Chest Cavity Exposure Tyrode's Solution With Heparin Lungs Removal Thymus Removal Inferior Vena Cava Removal Aorta Removal Heart Transfer Silicone Dissection Dish
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