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An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
 

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

Article DOI: 10.3791/54759-v • 07:23 min • October 10th, 2016
October 10th, 2016 •
1,2, 1, 1,2, 1, 1,2, 1, 1,2
1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 2Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology

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A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.

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Keyword Extraction: Radiolabeling Gold Nanoparticles 125I-labeled Azide Strain Promoted Copper Free Click Reaction Radiochemistry Radio Isotope Labeled Imaging Probes PET/SPECT Radiochemical Yield Radiochemical Purity Radio Iodination Reaction Chloramine T Sodium Metabisulfite HPLC Analysis Reversed Phase Analytical Radio HPLC Preparative Radio HPLC Radiochemical Yield
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