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Journal / Bioengineering / Mesenchymal Stromal Cell Culture and Delivery in…
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Bioengineering

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JoVE Journal Bioengineering
Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions
 

Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications

Article DOI: 10.3791/54845-v • 10:30 min • December 8th, 2016
December 8th, 2016 •
1,2, 2,3, 4, 1, 2,5, 2, 6, 1
1Dept. of Clinical and Experimental Medicine, University of Pisa, 2OtoLab, Azienda Ospedaliero-Universitaria Pisana (AOUP), 3Dept. of Civil and Industrial Engineering, University of Pisa, 4Immunohematology Operative Unit, Azienda Ospedaliero-Universitaria Pisana (AOUP), 5Dept. Of Surgical, Medical, Molecular Pathology and Emergency Medicine, University of Pisa, 6II Orthopedic and Traumatologic Clinic, Azienda Ospedaliero-Universitaria Pisana (AOUP)

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Culturing human Mesenchymal Stromal Cells (hMSCs) with autologous serum, reduces the risks of rejection by xenogeneic material and other negative effects. It also allows for the recovery of a subset of mesodermal progenitors, which can deliver fresh hMSCs. Embedding hMSCs in an autologous fibrin clot enables easy handling and effective surgical implantation.

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Keywords: Mesenchymal Stromal Cells Autologous Culture Fibrin Scaffold Orthopedic Applications Regenerative Medicine Tissue Engineering Bone Marrow Isolation Plasma Separation Clot Formation Cell Isolation Density Gradient Centrifugation
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