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Cancer Research

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JoVE Journal Cancer Research
Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies
 

Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies

Article DOI: 10.3791/54944-v • 09:53 min • November 30th, 2016
November 30th, 2016 •
1, 1, 1, 1, 1
1Department of Pathology, School of Medicine, University of Colorado Anschutz Medical Campus

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We describe a method for stable labeling of patient-derived xenografts (PDXs) with lentiviral particles expressing green-fluorescent protein and luciferase reporters. This method allows for tracking the growth of PDXs at the primary site, as well as detecting spontaneous and experimental metastases using in vivo imaging systems.

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Keywords: Breast Cancer Patient-derived Xenografts PDX Reporter Genes Tumor Growth Metastasis In Vivo Imaging Aseptic Technique Tissue Dissociation Cell Isolation Red Blood Cell Lysis
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