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Genetics

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JoVE Journal Genetics
Engineering Kunstmatige Factoren om specifiek manipuleren alternatieve splitsing in menselijke cellen
 
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Engineering Kunstmatige Factoren om specifiek manipuleren alternatieve splitsing in menselijke cellen

Article DOI: 10.3791/54967-v • 10:06 min • April 26th, 2017
April 26th, 2017 •
*1, *1, 2, 1, 1, 1, 1
1Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences (SIBS), 2Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center, Dalian Medical University
* These authors contributed equally

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Dit rapport beschrijft een werkwijze biotechnologie ontwerpen en bouwen van nieuwe kunstmatige splicingfactoren (ASFS) die specifiek de splitsing van doelgenen te moduleren in zoogdiercellen. Deze werkwijze kan verder worden uitgebreid met verschillende kunstmatige engineering factoren andere aspecten RNA metabolisme manipuleren.

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Genetica uitgave 122 kunstmatige RNA bindende factoren eiwit engineering splitsingsfactoren alternatieve splitsing PUF steiger RNA bindende eiwitten kanker
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