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Bioengineering

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JoVE Journal Bioengineering
An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage
 

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage

Article DOI: 10.3791/54984-v • 07:57 min • April 23rd, 2017
April 23rd, 2017 •
*1,2, *1,2, 1, 1,2,3
1Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology (TU Delft), 2Department of Orthopedics, UMC Utrecht, 3Department of Rheumatology, UMC Utrecht
* These authors contributed equally

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We propose a protocol to investigate the transport of charged and uncharged molecules across articular cartilage with the aid of recently developed experimental and numerical methods.

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Experimental Protocol Finite Element Transport Neutral Solutes Charged Solutes Articular Cartilage Physical Properties Diffusion Coefficient Fixed Charge Density Microcomputer Tomography Sample Preparation Cylindrical Osteochondral Plug Phosphate Buffered Saline (PBS) Heat Shrink Tubing Wet Cotton Ioxaglate Solution Iodixanol Solution Negatively Charged Solute Bath Neutral Solute Bath Sample Holder Motorized Stage Micro-CT
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