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Developmental Biology

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JoVE Journal Developmental Biology
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
 

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Article DOI: 10.3791/55196-v • 14:37 min • November 1st, 2017
November 1st, 2017 •
1, 1
1Department of Internal Medicine, Hematology Division, Washington University in St. Louis

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Here, we present human pluripotent stem cell (hPSC) culture protocols, used to differentiate hPSCs into CD34+ hematopoietic progenitors. This method uses stage-specific manipulation of canonical WNT signaling to specify cells exclusively to either the definitive or primitive hematopoietic program.

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Keywords: Human Pluripotent Stem Cells Hematopoietic Progenitors Directed Differentiation Primitive Hematopoiesis Definitive Hematopoiesis Stage-specific Signal Manipulation Embryoid Bodies Trypsin EDTA Wash Buffer HPSC Media Multigas Incubator
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