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Biochemistry

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JoVE Journal Biochemistry
Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
 

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Article DOI: 10.3791/55302-v • 09:43 min • May 5th, 2017
May 5th, 2017 •
1, 1, 1,2,3
1Department of Biology, Queen's University, 2Department of Biomedical and Molecular Sciences, Queen's University, 3School of Environmental Sciences, Queen's University

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This paper outlines the identification of ice-binding proteins from freeze-tolerant plants through the assessment of ice-recrystallization inhibition activity and subsequent isolation of native IBPs using ice-affinity purification.

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Keywords: Ice-binding Proteins IBPs Plant Freeze Tolerance Ice-affinity Purification Ice-recrystallization Inhibition Cold-acclimation Plant Extract Protein Extraction Centrifugation Ice-binding Activity
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