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Biochemistry

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JoVE Journal Biochemistry
Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
 

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Article DOI: 10.3791/55762-v • 09:13 min • June 14th, 2017
June 14th, 2017 •
1, 2, 1, 1, 1, 1, 2,3,4
1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan, Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section, University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit, Centro Cardiologico Monzino IRCCS

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The protein composition of the human mitral valve is still partially unknown, because its analysis is complicated by low cellularity and therefore by low protein biosynthesis. This work provides a protocol to efficiently extract protein for the analysis of the mitral valve proteome.

Tags

Optimized Protocol Extraction Of Proteins Human Mitral Valve Proteomic Analysis Pathogenic Mechanisms Cardiac Valve Disease Diagnostic Markers Prognostic Markers Therapeutic Targets Extraction Workflow Analytical Applications Exhaustive Characterization Porcine Mitral Valve Experimental Model Valve Function Evaluations Stefania Ghilardi Technician
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