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A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays
 

A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays

Article DOI: 10.3791/55854-v 07:11 min December 30th, 2017
December 30th, 2017

Capitoli

Riepilogo

The ability to accurately detect transcripts or proteins in Drosophila tissues is critical for studying their abundance and localization related to the development process. Here is the description of a straightforward procedure to dissect pupal wings. These wings can be used as samples in several techniques (immunohistochemistry, PCR assay, etc.).

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Keywords: Drosophila Pupal Wings Immunodetection PCR Assays Rapid Dissection Efficient Method Zero-hour APF Pupa Developmental Time Pupal Case Forceps Double-sided Tape Ventral Side Operculum Cuticle
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