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Journal / Genetics / Retroviral Scanning: Kortlægning af MLV…
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Genetics

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JoVE Journal Genetics
Retroviral Scanning
 
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Retroviral Scanning: Kortlægning af MLV Integration Sites til at definere Cell-specifikke Regulatory Regions

Article DOI: 10.3791/55919-v • 10:10 min • May 28th, 2017
May 28th, 2017 •
1,2, 3, 4, 3, 3, 3, 1,4, 1,2,4,5
1Center for Genome Research, Department of Life Sciences, University of Modena and Reggio Emilia, 2Laboratory of Chromatin and Gene Regulation During Development, Imagine Institute, 3Institute for Biomedical Technologies, CNR, 4Généthon, 5Sorbonne Paris Cité - Université Paris Descartes

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Her beskriver vi en protokol for genomgående kortlægning af integrationen af ​​Moloney-murine leukæmivirusbaserede retrovirale vektorer i humane celler.

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Genetik Udgave 123 Moloney leukæmi virus Integrationssteder Virus-vært interaktion Cis-regulerende elementer Epigenetisk regulering Cellidentitet Retroviral scanning Massiv sekventering
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