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Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood
 

Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood

Article DOI: 10.3791/56227-v • 07:08 min • September 5th, 2017
September 5th, 2017 •
1,2, 3, 4, 5, 5, 1
1Department of Clinical Research, University of Bern, 2Graduate School for Cellular and Biomedical Sciences, University of Bern, 3Immunology Research Centre, St. Vincent's Hospital Melbourne, 4Institute of Pathology, University of Bern, 5Institute of Molecular Animal Breeding and Biotechnology, Ludwig-Maximilian University

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We present an in vitro model which allows the study and analysis of coagulation in whole, non-anticoagulated blood. Anticoagulation in the system depends on the natural anticoagulation effect of healthy endothelial cells and endothelial cell activation will result in clotting.

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Keywords: 3D Cell Culture Endothelial Cells Anticoagulant Properties Anti-inflammatory Properties Whole Blood Non-anticoagulated Blood Cardiovascular Research Transportation Research Surgery Research Anti-immunity Endothelial Cell Activation Microcarrier Beads Coagulation Phosphate Buffered Saline (PBS) Dulbecco's Modified Eagle's Medium (DMEM) Medium 199 Trypsin EDTA Cell Culture Media Magnetic Stirrer Flask
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