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Immunology and Infection

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JoVE Journal Immunology and Infection
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
 

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Article DOI: 10.3791/56302-v • 11:25 min • October 4th, 2017
October 4th, 2017 •
*1, *1, *1, *1, 1, 1
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario
* These authors contributed equally

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Biochemical and structural analyses of glycosylated proteins require relatively large amounts of homogeneous samples. Here, we present an efficient chemical method for site-specific glycosylation of recombinant proteins purified from bacteria by targeting reactive Cys thiols.

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Keywords: Cysteine Thiols Site-specific Glycosylation Recombinant Proteins Structural Biology Glycosylation Patterns Disease Therapy PCR Mutagenesis DPN1 Digestion Protein Expression
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