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Bioengineering

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JoVE Journal Bioengineering
Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae
 

Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae

Article DOI: 10.3791/56340-v • 14:05 min • October 27th, 2017
October 27th, 2017 •
*1,2, *3, 2, 2, 4, 5,6, 1,2,3
1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Morgridge Institute for Research, University of Wisconsin-Madison, 3Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 4Cellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison, 5Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 6Department of Pediatrics, University of Wisconsin-Madison
* These authors contributed equally

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Automatic Translation

This manuscript describes the zWEDGI (zebrafish Wounding and Entrapment Device for Growth and Imaging), which is a compartmentalized device designed to orient and restrain zebrafish larvae. The design permits tail transection and long-term collection of high-resolution fluorescent microscopy images of wound healing and regeneration.

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Keywords: Zebrafish Larvae Wound Healing Live Imaging PDMS 3D Printing Microscopic Analysis Collagen Fiber Reorganization Cellular Responses Tissue Regrowth ZWEDGI PDMS Fabrication Mold Cleaning UV Postcuring Sandpaper Ultrasonic Cleaning Isopropyl Alcohol PDMS Casting Vacuum Degassing
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