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Bioengineering

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JoVE Journal Bioengineering
A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans
 

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

Article DOI: 10.3791/63136-v • 10:45 min • April 11th, 2022
April 11th, 2022 •
1,2,3,4, 1,5, 2
1National Centre for Biological Sciences, TIFR, 2Department of Biological Sciences, TIFR, 3InSTEM, 4Manipal Academy of Higher Education, 5Department of Mechanical Engineering, The University of Texas at Austin

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The protocol describes a simple microfluidic chip design and microfabrication methodology used to grow C. elegans in presence of a continuous food supply for up to 36 h. The growth and imaging device also enables intermittent long-term high-resolution imaging of cellular and sub-cellular processes during development for several days.

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Microfluidic Chip Caenorhabditis Elegans Device Fabrication Flow Layer Control Layer Photoresist Spin Coating UV Exposure Soft Bake Device Reusability Imaging Long-term Growth
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