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Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
 

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Article DOI: 10.3791/56580-v • 09:05 min • February 23rd, 2018
February 23rd, 2018 •
1, 1, 2, 2, 3,4, 3, 1, 1, 1, 5, 5, 4,6, 1
1CEA, LETI, DTBS, LISA, Université Grenoble Alpes, 2CEA, LETI, DTBS, LBAM, Université Grenoble Alpes, 3CEA, INSERM, BIG, Université Grenoble Alpes, 4CNRS, FR CNRS 3425, 5CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport, PSL Research University, Institut Curie, 6TIMC-IMAG

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Lens-free video microscopy enables us to monitor cell cultures directly inside the incubator. Here we describe the full protocol used to acquire and analyze a 2.7 day long acquisition of cultured HeLa cells, leading to a dataset of 2.2 x 106 measurements of individual cell morphology and 10584 cell cycle tracks.

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Keywords: Lens-free Video Microscopy Adherent Cell Culture Label-free Time-lapse Acquisition Holographic Reconstruction Hela Cells Fibronectin Coating Incubator-compatible CMOS Image Sensor LED Illumination
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