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Bioengineering

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JoVE Journal Bioengineering
Methodology for Biomimetic Chemical Neuromodulation of Rat Retinas with the Neurotransmitter Glutamate In Vitro
 

Methodology for Biomimetic Chemical Neuromodulation of Rat Retinas with the Neurotransmitter Glutamate In Vitro

Article DOI: 10.3791/56645-v • 12:56 min • December 19th, 2017
December 19th, 2017 •
1, 2, 1
1Department of Mechanical and Industrial Engineering, University of Illinois at Chicago, 2Department of Biomedical Engineering, Northwestern University

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This protocol describes a novel method for investigating a form of chemical neurostimulation of wholemount rat retinas in vitro with the neurotransmitter glutamate. Chemical neurostimulation is a promising alternative to the conventional electrical neurostimulation of retinal neurons for treating irreversible blindness caused by photoreceptor degenerative diseases.

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Methodology Biomimetic Chemical Neuromodulation Rat Retinas Neurotransmitter Glutamate In Vitro Artificial Vision Photoreceptor Degenerated Retina Visual Acuity Naturalistic Vision Electrical Stimulation Blindness Degenerative Diseases Therapeutic Intervention Glutamate Sensitivity Enucleated Eyes Petri Dish Oxygenated Ames' Medium Solution Dissection Stereo Microscope Corneal Incision Sharp Scissors Detached Cornea
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