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Biochemistry

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JoVE Journal Biochemistry
Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
 

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay

Article DOI: 10.3791/56846-v • 10:41 min • March 7th, 2018
March 7th, 2018 •
1, 2, 1, 3
1Department of General Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 2Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine, Shanghai Jiao Tong University, 3Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine

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The protocol here describes the interactions of purified hEAG1 ion channel protein with the small molecule lipid ligand phosphatidylinositol 4, 5-bisphosphate (PIP2). The measurement demonstrates that BLI could be a potential method for novel small-molecule ion channel ligand screening.

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Bio-layer Interferometry Assay Interaction Kinetics Ion Channel Protein Small Molecules HEAG1 Ion Channel Pharmacology High Throughput Method Immobilized Protein Protein Expression Purification Labeling Sensor Tuning Data Analysis HEK293 T Cells Cell Culture Lysis Buffer
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