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Cancer Research

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JoVE Journal Cancer Research
Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
 

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology

Article DOI: 10.3791/56943-v • 11:20 min • March 21st, 2018
March 21st, 2018 •
1,2, 1, 2, 1,3
1Genome Analysis Center, Yamanashi Central Hospital, 2Pathology Division, Laboratory Department, Yamanashi Central Hospital, 3The University of Tokyo

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Obtaining high-quality genomic DNA from tumor tissues is an essential first step for analyzing genetic alterations using next generation sequencing. In this article, we present a simple and rapid method to enrich tumor cells and obtain intact DNA from touch imprint cytology specimens.

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Keywords: Tumor DNA Touch Imprint Cytology Clinical-pathological Specimens High-quality DNA Rapid DNA Extraction Cancer Research Diagnosis Treatment DNA Extraction Microscopic Assessment DNA Sequencing
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