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Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
 

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

Article DOI: 10.3791/57153-v • 13:27 min • March 31st, 2018
March 31st, 2018 •
1, 1, 2, 1, 1, 1,3, 4,5, 1, 1,6, 1,7
1School of Biological Sciences, The University of Auckland, 2Research School of Biology, The Australian National University, 3Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, 4Department of Plastic Surgery, Counties Manukau District Health Board, 5Department of Surgery, Faculty of Medical and Health Sciences, The University of Auckland, 6Biomedical Imaging Research Unit, Faculty of Medical and Health Sciences, The University of Auckland, 7Maurice Wilkins Centre, The University of Auckland

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Traditional methods of assessing adipogenic differentiation are cheap and easy to use, but are not specific to changes in gene expression. We have developed an assay to quantify mesenchymal cell differentiation into mature adipocytes using a lineage specific marker. This assay has diverse applications across basic research and clinical medicine.

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Keywords: Mesenchymal Cell Adipogenic Differentiation Lineage-specific Marker Fatty Acid Binding Protein 4 Stromal Cells High-content Screening Immunofluorescent Microscopy Image Analysis Oil Red O Gene Expression Drug Screening 96-well Plate Confluence Differentiation Medium
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