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Immunology and Infection

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Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

Article DOI: 10.3791/57171-v • 08:24 min • February 27th, 2018
February 27th, 2018 •
1, 2, 1, 1, 1, *1, *1
1Department of Biology, Middle Tennessee State University, 2DRVision Technologies
* These authors contributed equally

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The polysaccharide capsule is the primary virulence factor in Cryptococcus neoformans, and its size correlates with strain virulence. Capsule diameter measurements are used in phenotypic testing and to gauge therapeutic efficacy. Here a standard method of capsule induction is presented, and two methods of staining and measuring diameter are compared.

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Keywords: Cryptococcus Neoformans Capsule Diameter Capsule Staining India Ink Calcofluor-white Alexa Fluor 488 Capsule Measurement Virulence Automated Analysis
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