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Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
 

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Article DOI: 10.3791/57240-v • 09:23 min • June 29th, 2018
June 29th, 2018 •
*1,2, *1, 1,2, 1,2, 3, 2, 1
1Department of Biological Sciences, University of South Carolina, 2Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, 3Department of Instructions and Teacher Education, University of South Carolina
* These authors contributed equally

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Citrus Greening is a particularly destructive disease affecting citrus crops globally. Presented here is a simple method using PCR and genomic DNA extraction of citrus leaf tissue for the accurate and precise identification of the citrus greening pathogen, Candidatus liberibacter spp.

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Keywords: Citrus Greening Candidatus Liberibacter Spp. Citrus Leaf Tissue PCR Detection Pathogen Identification Citrus Farming Quantitative Analysis Laboratory Procedure RNA Removal DNA Precipitation Centrifugation
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