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Developmental Biology

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JoVE Journal Developmental Biology
A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes
 

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

Article DOI: 10.3791/57556-v • 10:56 min • September 15th, 2018
September 15th, 2018 •
*1,2,6, *2, *3, 1,2, 2, 2, 4,5, 4,5, 2, 4,5, 4,5, 4,5, 4,5, 3, 4,5,7, 1,2,6,7
1Department of Medicine, University of Hong Kong-Shenzhen Hospital, 2The Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, 3School of Biomedical Sciences, Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, 4Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University, 5Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 6Research Centre of Heart, Brain, Hormone, and Healthy Ageing, Li Ka Shing Faculty of Medicine, University of Hong Kong, 7Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre, University of Hong Kong and Guangzhou Institutes of Biomedicine and Health
* These authors contributed equally

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Here, we present a protocol to generate a human liver chimeric mouse model of familial hypercholesterolemia using human induced pluripotent stem cell-derived hepatocytes. This is a valuable model for testing new therapies for hypercholesterolemia.

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Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Induced Pluripotent Stem Cells Hepatocytes Drug Tests In Vivo Treatments Chimeric Animal Models Inherited Liver Diseases Extracellular Matrix Insulin Syringe DNAase 1 RPMI 1640 Medium Cell Dissociation Enzyme Phase Contrast Images Cell Morphology Growth Cell Density
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