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Biochemistry

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JoVE Journal Biochemistry
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
 

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Article DOI: 10.3791/57801-v • 14:23 min • June 6th, 2018
June 6th, 2018 •
1, 1, 1
1Molecular Physiology and Biophysics, University of Iowa

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Batch processing of yeast 2-hybrid screens allows for direct comparison of the interaction profiles of multiple bait proteins with a highly complex set of prey fusion proteins. Here, we describe refined methods, new reagents, and how to implement their use for such screens.

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Keywords: Yeast 2-hybrid Protein-protein Interactions Deep Sequencing Bait Plasmid Prey Library MATa MATα Centromere Kanamycin Gal4 DNA Binding Domain Immunoblotting Genomic DNA Adapter CSM Medium
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