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Biochemistry

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JoVE Journal Biochemistry
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
 

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Article DOI: 10.3791/62949-v • 06:07 min • August 5th, 2022
August 5th, 2022 •
1, 2, 2
1Department of Chemistry, Bucknell University, 2Program in Cell Biology/Biochemistry, Bucknell University

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DNA methyltransferases are potential cancer drug targets. Here, a protocol is presented to assess small molecules for DNA methyltransferase inhibition. This assay utilizes an endonuclease to couple DNA methylation to fluorescence generation and allows for enzyme activity to be monitored in real time. 

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Keywords: Continuous Fluorescence-based Assay Endonuclease-coupled DNA Methyltransferase Inhibitors Plate Reader Assay Real-time Data Collection DNA Methylation SAM GlaI DNMT1 DNA Substrate Inhibitor Screening
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