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JoVE Journal Biochemistry
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
 

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Article DOI: 10.3791/59737-v • 08:04 min • October 8th, 2019
October 8th, 2019 •
1, 1, 2, 2, 1, 1
1Department of Chemistry, Ben-Gurion University of the Negev, 2Center for Genomic and Computational Biology, Department of Biostatistics and Bioinformatics, Duke University

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Protein binding microarray (PBM) experiments combined with biochemical assays link the binding and catalytic properties of DNA primase, an enzyme that synthesizes RNA primers on template DNA. This method, designated as high-throughput primase profiling (HTPP), can be used to reveal DNA-binding patterns of a variety of enzymes.

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Keywords: DNA Primase Protein-DNA Binding Microarray High-throughput Sequence Recognition RNA Primer Formation Transcription Factors DNA Binding Enzymatic Activity Sequence Determinants
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