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Biochemistry

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JoVE Journal Biochemistry
Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
 

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Article DOI: 10.3791/63501-v • 06:32 min • March 9th, 2022
March 9th, 2022 •
1, 1, 1, 1,2
1Department of Biological Sciences, Ulsan National Institute of Science and Technology, 2Center for Genomic Integrity, Institute for Basic Science (IBS)

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DNA curtain, a high-throughput single-molecule imaging technique, provides a platform for real-time visualization of diverse protein-DNA interactions. The present protocol utilizes the DNA curtain technique to investigate the biological role and molecular mechanism of Abo1, a Schizosaccharomyces pombe bromodomain-containing AAA+ ATPase.

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Keywords: DNA Curtain Single-molecule Imaging Chromatin Dynamics Histone Chaperones TIRF Microscopy Biomolecule Interactions Lambda DNA Streptavidin Biotinylated Lipid YOYO-1 Dye Microfluidic System
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