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Biochemistry

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JoVE Journal Biochemistry
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
 

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Article DOI: 10.3791/61707-v • 07:10 min • March 4th, 2021
March 4th, 2021 •
1, 1, 2, 2, 2, 1, 1, 1, 3, 3, 3, 4,5, 6, 6, 1, 6, 1, 1
1Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute, Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center, University of California Agriculture and Natural Resources, 4West Side Research and Extension Center, University of California, 5Department of Plant Sciences, University of California, Davis, 6Department of Plant and Microbial Biology, University of California, Berkeley

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The protocol has been developed to effectively extract intact histones from sorghum leaf materials for profiling of histone post-translational modifications that can serve as potential epigenetic markers to aid engineering drought resistant crops.

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Isolation Histone Sorghum Leaf Tissue Top-down Mass Spectrometry Profiling Epigenetic Markers Molecular Understanding Crops Extreme Climates High Purity Post-translational Modifications Procedure Grind Liquid Nitrogen Centrifuge Tube Extraction Buffers Protease Inhibitor Tablet Concentration Ground Leaf Powder Mix Vortex Mesh 100 Rinse Filtrate Centrifuge Swinging Bucket Rotor
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